Type: Chapter

Sequencing and assembly of the wheat genome


Kellye Eversole


Jane Rogers


Beat Keller

University of Zurich

Rudi Appels

Murdoch University

Catherine Feuillet

Bayer CropScience

Publication date:

27 June 2017

ID: 9781786760166-004

E-Chapter format

£25.00 excluding tax


The genome sequence is an essential prerequisite for defining the complete gene catalogue of an organism, and it provides a framework for identifying and understanding the function of variants associated with phenotypic traits of interest. For wheat, these traits include disease resistance, crop yield improvement, protein content, quality factors and resistance to abiotic stressors such as drought or poor soils. This chapter reviews the challenges posed by the structure and composition of the wheat genome and how they are being addressed through genome sequencing strategies. It describes the sequencing approaches and resources that have been generated to date and provides some examples of how they are used. The chapter also discusses the road map of the International Wheat Genome Sequencing Consortium for the completion of a reference sequence that will meet the needs of breeders and researchers seeking to improve crop varieties and to understand the dynamic genome environment and complexity of the world’s most important crop.

Table of contents

1 Introduction
2 Challenges of the wheat genome
3 Sequencing technologies and strategies
4 Characterizing the wheat genome sequence
5 Strategies to obtain a reference sequence of the bread wheat genome
6 Strategies for sequencing the bread wheat genome
7 Chromosome-based sequencing for wheat
8 Delivering a reference sequence of the bread wheat genome: a road map
9 A chromosome-based survey sequence of the 21 bread wheat chromosomes
10 BAC MTP sequencing of the 21 bread wheat chromosomes
11 Towards the reference sequence of wheat
12 Future trends and conclusion
13 Where to look for further information
14 References