Type: Book

Genome editing for precision crop breeding


Dr Matthew R. Willmann is Director of the CALS Plant Transformation Facility (PTF) at Cornell University, USA. The Facility produces transgenic and CRISPR/Cas9 genome-edited plants such as rice, maize, wheat and apple, as well as researching new genome editing techniques. With 25 years of experience of plant science research, Dr Willmann has published widely in such areas as gene editing and is on the editorial board of The CRISPR Journal which promotes research in this important and growing area.



Publication date:

23 March 2021

Length of book:

440 pages

ISBN-13: 9781786764478

Hardback - £180.00
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Genome editing is rapidly transforming plant research. The technique offers unparalleled precision in breeding without the need to introduce foreign DNA into plants. CRISPR/Cas systems have established themselves as the leading technique in genome editing. This collection takes stock of the wealth of research on these techniques and their potential in crop breeding in improving traits such as yield, disease resistance, drought tolerance and nutrient use efficiency.

Part 1 of this volume reviews advances in gene editing techniques such as TALENS and zinc finger nucleases, double-strand break repair techniques, insertion-based genome edits, base editing, guide RNAs, gRNA/Cas9 constructs and CRIST/Cas off targeting. Chapters also discuss advances in screening plants as well as regulatory issues. Part 2 surveys applications of gene editing in key cereal including wheat, barley, maize, rice and sorghum. Other chapters cover crops such brassicas, potato and tomato.

Key features

  • Comprehensive, systematic review of advances in key CRISPR/Cas technologies, such as TALENS and zinc finger nucleases, double-strand break repair techniques, insertion-based genome edits, base editing, guide RNAs, gRNA/Cas9 constructs and CRIST/Cas off targeting
  • Covers both techniques and their practical application to particular cereal and other crops 
  • Discusses challenges in regulating this emerging technology

What others are saying...

"CRISPR/Cas technology is revolutionizing molecular biology and has the potential to create a novel, more sustainable agriculture. With its breadth of coverage and the expertise of the distinguished international team of contributing authors, this comprehensive guide to genome editing for precision crop breeding promises to be an ideal reference for researchers and graduate students and those working in agriculture and plant biotechnology around the world." Prof. Caixia Gao, Institute of Genetics and Developmental Biology – Chinese Academy of Sciences

Table of contents

Part 1 Genome editing techniques
1.Using TALENS and zinc finger nucleases as genome-editing reagents in plants: Adam Bogdanove, Cornell University, USA;
2.Double-strand break (DSB) repair in plants: Holger Puchta, Botanical Institute II - Karlsruhe Institute of Technology, Germany;
3.Advances in the generation of insertion-based genome edits in plants: Qing-Hui Yu, Xinjiang Chinese Academy of Agricultural Sciences, China;
4.Viruses as vectors for the delivery of gene-editing reagents: Evan E. Ellison, James C. Chamness and Daniel F. Voytas, University of Minnesota, USA;
5.Base editing in plants: Nathaniel Graham, Pairwise Plant, USA;
6.Advances in designing guide RNAs in genome editing of plants: Ling-Ling Chen, Huazhong Agricultural University, China;
7.Advances in assembling gRNA/Cas9 constructs in genome editing of plants: Diego Orzaez, Polytechnic University of Valencia, Spain;
8.Advances in delivering genome editing constructs into plant cells: William Gordan-Kamm, Corteva, USA;
9.Advances in screening plants for edits and off targets: Chung Wang, Chinese Academy of Agricultural Sciences, China;
10.Genome editing on promoter regions: Andika Gunadi and Ning Zhang, Boyce Thompson Institute, USA; and John J. Finer, The Ohio State University, USA;
11.The regulation of genome-edited crops: Gregory Jaffe, Center for Science in the Public Interest, USA;

Part 2 Applications
12.Genome editing of barley: Martin Becker, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Germany; and Goetz Hensel, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Germany and Palacký University, Czech Republic;
13.Genome editing of maize: Kan Wang, Iowa State University, USA;
14.Genome editing of sorghum: David Holding, University of Nebraska-Lincoln, USA;
15.Genome editing of brassica crops: Cheng Dai, Huazhong Agricultural University, China;
16.Genome editing of tomatoes and other Solanaceae: Joyce Van Eck, Boyce Thompson Institute – Cornell University, USA;
17.Genome editing of perennial crops: Chung Jui (CJ) Tsai, University of Georgia, USA;